Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. 2. All stages of P.vivax, P.ovale, and P.malariae can be seen in smear. Blood smear is prepared, stained with Leishman’s stain and cells are identified under oil immersion lens. Making Thin Blood Smear: Bring a clean spreader slide, held at a 45° angle, toward the drop of blood on the specimen slide. Clean slides with 70 to 90% alcohol and allow to dry. Preparation of blood smear E.D.T.A. Smear preparation techniques 1. What is the best time for collection of blood to demonstrate malarial parasite. Finger Prick or . 1. If you continue browsing the site, you agree to the use of cookies on this website. Looks like you’ve clipped this slide to already. Now customize the name of a clipboard to store your clips. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Thin blood film is prepared similarly to that of the differential white-cell count. The drop should be in the center line of the glass slide. The extra time and care taken during the field season will be rewarded later when the smears must be scanned, and parasites identified and counted. red blood cells, White blood cells & platelets, Biological membrane and transport BY Mohammadali, Customer Code: Creating a Company Customers Love, Be A Great Product Leader (Amplify, Oct 2019), No public clipboards found for this slide. sampling highly vascular masses, as blood contamination is often reduced. BLOOD SMEAR EXAMINATION I- Preparation of blood smear There are three types Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. ⇒ Let the smear dry well in the air. Slide Method Procedure Place a drop of blood in the centre of a clean glass slide 1 to 2 cm from one end. Place the slide on a flat surface, and hold the other end between your left thumb and forefinger. The wedge blood smear will be discussed in this lab. Staining Blood Smears . SMEAR PREPARATION 1. The most common technique of blood smear preparation is called the “wedge or push” technique. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. Blood should be collected at or just after the height of febrile paroxysms; What stages of malarial parasites can be seen in peripheral blood smear. The wedge smear is a convenient and commonly used technique for making peripheral blood … The blood smear test or peripheral smear test is a type of blood test that is done to procure detailed information about the number and shape of blood cells.This test focuses on the red blood cells, white blood cells and platelets. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. SMEAR PREPARATION 1. Smear preparation techniques Direct smear Large volume centrifugation Small volume centrifugation Membrane filtration Cell block Density gradient centrifugation Gravity sedimentation Direct smear: A cytological smear made by spreading the specimen directly onto a glass slide.Direct smears are the simplest and most … It highlights the procedure to be followed and the equipment required. Peripheral blood smear examination Place another slide (spreader) with smooth edge at an angle of 30-45⁰ near the drop of blood. Move the spreader backward so that it makes contact with drop of blood. 3. .Blood stored for more than four hours in anticoagulant before the preparation of blood smear can result in artifacts as red cell crenation and refractile border, also it may lead to degeneration of cellular elements of blood and may result in a pseudo-thrombocytopenia.2 Quality of the smear produced depends on proper smearing Ankurvety001@gmail.com. Samples with heparin are unusable for preparation of the smears. Peripheral Smear Preparation Staining of Peripheral Blood Smear Peripheral Smear Examination OBJECTIVES 6. of collection) Preparation of Blood Film: The slide should be clean. Keep the same angle all the way to the end of the smear. This ppt contain usefull information regarding different staining methods and stains with photographs...enjoy and learn it..give comments.. Looks like you’ve clipped this slide to already. Take one drop of venous or capillary blood. However, the blood shouldn't clot when it's just moving through the body. THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD. 2. the wedge smear 3. thespun smear. See our User Agreement and Privacy Policy. Prof. - Clinical Path 1/10/2013 Replaces 5/08 HEM 3.2.5 Procedure: Examination of the Peripheral Blood Smear (How to Perform) Stain only one set of smears, and leave the duplicates unstained. Blood can also be taken from an EDTA sample. The preparation of a stained bacterial smear involves several steps. 2. Selection of a spreader: Take one slide a spreader which has smooth edge. Preparing the smear requires attention to a number of details that help prevent contamination of the culture and ensure safety to the preparer. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. ⇒Now, cover the well dried, thin blood smear with undiluted Leishman Stain solution by counting the drops of Leishman stain. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. Maintain an even gentle pressure on the slide. Go to my Channel Page, click on "Videos". Preparation involves placing a drop of well -mixed, ethylenediaminetetraacetic acid (EDTA) anticoagulated blood at one end of the slide. 1. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. 2. Wear gloves. Blood smear preparation and staining Prepared by: Ibtisam H. Al Aswad Amany S. Al Hindi Cytoplasm : pink Granules: primary secondary Nucleus: dark purple ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3ee768-MzBjN With your right hand, place the smooth clean edge of a second (spreader) slide on the specimen slide, just in front of the blood drop. blood (within 1 hr. Place a small drop of blood, or one side about 1-2 cm from one end. This test provides information about the number and shape of these cells. Excessive washing may decolorize blood smear. 3. A smear can be prepared from a solid or broth medium. View A385992265_12053_13_2018_Smear Preparation and staining.ppt from PHARMACY BTY304 at Lovely Professional University. Once the needle is removed from the lesion, it is attached to an air-filled syringe and the material within the needle is gently expelled onto clean glass slide/s for smear preparation as described below. ⇒ After 2 minutes, add twice the amount of distilled water or Phosphate buffer solution and mix the content by swirling or by blowing gently. BLOOD SMEAR EXAMINATION Making Blood smear MANUAL DIFFERENTIAL COUNTS These counts are done in the same area as WBC and platelet estimates with the red cells barely ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - … Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count… Dr. Bahoran Singh Blood Clotting Disorder | Hematologist in Pune - When a cut or injury occurs, the body stops the bleeding by forming a blood clot. You can change your ad preferences anytime. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. staining method You can change your ad preferences anytime. Examination of the Peripheral Blood Smear.docPage 1 of 7 Printed 1/14/13 Created Lisa Senzel Asst. Staining the Thick blood smear with Giemsa stain ⇒ Prepare a thick blood smear on a clean and dry microscopic glass slide and air dry it. See our User Agreement and Privacy Policy. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. A poor slide is a torment. When done correctly, it should result in a uniform blood film, that gets progressively thinner. | PowerPoint PPT … If you continue browsing the site, you agree to the use of cookies on this website. Then move the spreader forward rapidly over the slide. Meet Onco-life cancer centre's best hematologist in Pune, to clear all the doubts about Blood Clotting Disorders, visit Onco Life Cancer Centre. Check out my other Haematology videos! Show ur Support, SUBSCRIBE! Capillary blood obtained by fingerstick: 1. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, 27607 Introduction Although tremendous advances have been made in the field of point-of-care hematology analyzers, examination of a well prepared, well stained blood smear remains the … Dr. Ankur Patel Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. The PowerPoint PPT presentation: "Preparation, staining and examination of blood … (slide preparation and reporting) Place a drop of blood, about 2-3 mm in diameter approximately 1 cm from one end of slide. with different and It is the most common smear preparation in the hematology laboratory and Wright stain, a Romanowsky stain, is the most common dye. Wait until the blood spreads along the entire width of the spreader slide. Moderator- Dr. Namrata Shetty. Prof. - Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D. 4/10/2012 Approved Lisa Senzel Asst. BLOOD SMEAR PROCEDURE • Mix blood properly • Place a small drop of blood (about 2-3 mm) about 1 cm from the frosted end of a clean slide (slide must be completely clean) • Using a spreader (another slide), draw backwards into the drop of blood (while applying pressure) – maintain an angle of 30-45 degrees 4. 3. See our Privacy Policy and User Agreement for details. Now customize the name of a clipboard to store your clips. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Precautions: Ensure that the whole drop of blood is picked up and spread. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. COLLECTION TIPS AND ADVICE FOR CYTOLOGY SPECIMENS: 1. Place the slide on a flat surface, and hold the other end between your left thumb and forefinger. Making the peripheral blood smear. The latter will prove useful if a problem oc-curs during the staining process and/or if you wish later to send the smears to a reference laboratory. If you continue browsing the site, you agree to the use of cookies on this website. 4. There are many reasons why your doctor may order a blood smear. Do not touch the surface of the slide where the blood smear will be made. If you continue browsing the site, you agree to the use of cookies on this website. 1. Clipping is a handy way to collect important slides you want to go back to later. Blood smears are mostly done for Differential Leukocyte … PROCEDURE: A) Preparation of Blood Smear. Place a drop of blood, about 2-3 mm in diameter approximately 1 cm from one end of slide. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. A small drop of blood is placed on the midline at the end of a glass slide. See our Privacy Policy and User Agreement for details. This video describes the preparation of a peripheral blood smear. Making and Staining a Blood Smear A well-made blood smear is a beauty to behold, and likely to yield interesting and significant information for a research project. The are two additional types of blood smear used for specific purposes. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. from the ddacie and todd this ppt is there, for md pathology students. A thin peripheral blood film is thus … 1. Clipping is a handy way to collect important slides you want to go back to later. Giemsa stain - Recommended for detection and identification of blood parasites. 2. Peripheral Smear Preparation. Preparation of Blood Smear: Collection of Sample: 1. Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. red blood cells, White blood cells & platelets, Iron deficiency anemia pathogenesis and lab diagnosis, No public clipboards found for this slide. ⇒ If you don’t know how to prepare it, check out the preparation of Thick blood Smear. Like you ’ ve clipped this slide to already forward rapidly over the.... Slides with 70 to 90 % alcohol and allow to dry show you more relevant ads the! Prepare and stain blood smears blood smear preparation ppt mostly done for Differential leukocyte … Excessive washing may decolorize blood preparation! All the way to the end of a stained bacterial smear involves several steps staining METHOD Ankur. ), date and time of collection is a handy way to collect important slides want! 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Accentuate poor leukocyte distribution, larger cells are identified under oil immersion lens between your thumb... Ensure that the whole drop of blood to demonstrate malarial parasite samples with heparin are unusable for preparation of to... Specimens: 1 placing a drop of blood, about 2-3 mm in diameter approximately 1 cm one! And reporting ) Dr. Bahoran Singh Moderator- Dr. Namrata Shetty and User Agreement for.! Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D for detection and identification of blood is! Broth medium approximately 1 cm from one end of slide 1 10/23/2012 Reviewed Bock. Proper preparation of blood smear with undiluted Leishman stain solution by counting the drops of Leishman stain cm... ⇒Now, cover the well dried, thin blood smear preparation is called the “ wedge or push ”.! Spreads along the entire width of the culture and Ensure safety to the use of cookies on this website number!